Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences TrizmaR base Primary Stand25KG
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations such as Tris-EDTA (TE) buffer Tris-acetate-EDTA (TAE) buffer Tris-borate-EDTA (TBE) buffer etc. It is pure essentially stable relatively non-hygroscopic and has a high equivalent weight.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate 99.5 titration50KG
MES is one of the Good buffers the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes.
Medchemexpress LLC MOPSO sodium | 79803-73-9 | 97.0% | 247.24 | C7H14NNaO5S | 500 G
MOPSO sodium is the sodium salt of MOPSO, a zwitterionic buffering agent used in biochemical and molecular biology applications to prepare buffer solutions.
- Used for preparation of buffer solutions.
- Zwitterionic buffering agent; effective pH range typically near physiological pH.
- CAS number 79803-73-9.
- Chemical formula C7H14NNaO5S; molecular weight 247.24.
- Purity approximately 97.0% and available in 500 g packages.
Electron Microscopy Sciences Tris Buffered Saline, pH 7.4 500 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 7.4
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 25g
BICINE | Purity: ≥99% (titration) | MW:163.17 | 150-25-4 | MFCD00004295 | 25g
![Research Products International Corp TBE Buffer [TRIS-Borate-EDTA], 10X Powder, 170.3 Grams of Powder Makes 1 Liter of Solution](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T22020-1.0.JPG-250.jpg)
Research Products International Corp TBE Buffer [TRIS-Borate-EDTA], 10X Powder, 170.3 Grams of Powder Makes 1 Liter of Solution
Dry homogeneous blend of molecular biology grade TRIS base, Boric Acid and EDTA.
For use in gel electrophoresis.
Teknova 10mM Tris-HCl, pH 8.5. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Electron Microscopy Sciences Hepes, Crystal, Biological Buffer, 7.0-8.0 pH 25 GR
(N-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic acid)
CAS #7365-45-9 C₈H₁₈O₄N₂S F.W. 238.30
Assay: >99%
Good's buffer.
pH: 7.0-8.0
Electron Microscopy Sciences Hepes, Crystal, Biological Buffer, 7.0-8.0 pH 100 GR
(N-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic acid)
CAS #7365-45-9 C₈H₁₈O₄N₂S F.W. 238.30
Assay: >99%
Good's buffer.
pH: 7.0-8.0
General Laboratory Products 2mL 0.85 per. Saline, 100/cs
2mL 0.85 per. Saline with Shelf Life of 1 yr.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml.
eMolecules tert-butyl 3-(hydroxymethyl)-4-methyl-pyrrolidine-1-carboxylate | 1184985-22-5 | MFCD08461237 | 1g
Pharmablock | tert-butyl 3-(hydroxymethyl)-4-methyl-pyrrolidine-1-carboxylate | 1g | 551098866 | PB96772 | | 1184985-22-5 | MFCD08461237 | 215.293 | C11H21NO3
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